ELA, Volume 8, pp. 128-139, August 2001, abstract. The third smallest eigenvalue of the Laplacian matrix Sukanta Pati Let G be a connected simple graph. The relationship between the third smallest eigenvalue of the Laplacian matrix and the graph structure is explored. For a tree the complete description of the eigenvector corresponding to this eigenvalue is given and some results about the multiplicity of this eigenvalue are given.